RT Journal Article
SR Electronic
T1 Differential Induction by 2,3,7,8-Tetrachlorodibenzo-<em>p</em>-Dioxin of Multiple Forms of Rabbit Microsomal Cytochrome <em>P</em>-450: Evidence for Tissue Specificity
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 565
OP 570
VO 18
IS 3
A1 LIEM, HENG H.
A1 MULLER-EBERHARD, URSULA
A1 JOHNSON, ERIC F.
YR 1980
UL http://molpharm.aspetjournals.org/content/18/3/565.abstract
AB Four forms of cytochrome P-450, forms 2, 3, 4, and 6, are presently being isolated and characterized in this laboratory. Induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin of specific forms of microsomal cytochrome P-450 was studied in adult rabbit kidney and lung. The induction of a single prominent cytochrome P-450 form was observed; this cytochrome was identified as form 6. The assigmnent of the inducible pulmonary and renal cytochrome as form 6 was established by its mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, its peptide map, and its specific antigenicity. In contrast, previous findings have shown that form 4 is the major cytochrome P-450 induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in adult rabbit liver. Thus, the specific induction of cytochrome P-450 forms 4 and 6 is tissue dependent. ACKNOWLEDGMENTS The technical assistance of Maria Balga, Maryann Zounes, and Kendis Cox is gratefully acknowledged. We wish to thank Diane Montoya and Pat Matson for preparation of the manuscript.