PT  - JOURNAL ARTICLE
AU  - COOPER, DERMOT M. F.
AU  - LONDOS, CONSTANTINE
AU  - RODBELL, MARTIN
TI  - Adenosine Receptor-Mediated Inhibition of Rat Cerebral Cortical Adenylate Cyclase by a GTP-Dependent Process
DP  - 1980 Nov 01
TA  - Molecular Pharmacology
PG  - 598--601
VI  - 18
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/18/3/598.short
4100  - http://molpharm.aspetjournals.org/content/18/3/598.full
SO  - Mol Pharmacol1980 Nov 01; 18
AB  - Adenosine analogues inhibit rat cerebral cortical adenylate cyclase by a receptor-mediated GTP-dependent process. N6-Phenylisopropyladenosine and other analogues of adenosine are potent inhibitors (Ki values of 10-100 nM) of adenylate cyclase in a particulate preparation of rat cerebral cortex. The detection of such inhibitory effects is facilitated by the use of a deoxy ATP assay system. The inhibition is strictly dependent on GTP, is amplified by Na+ ions, and is antagonized by methylxanthines. ACKNOWLEDGMENTS The authors wish to thank Drs. John Daly and Bill Padgett for helpful discussions and advice on membrane preparations. The technical assistance of Mr. James Oden and the expert secretarial assistance of Ms. Bonnie Richards are also gratefully acknowledged.