RT Journal Article
SR Electronic
T1 Effects of Nethalide on Insulin Activity and Binding by Rat Muscle and Adipose Tissue
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 227
OP 236
VO 2
IS 3
A1 P. D. BEWSHER
A1 C. C. HILLMAN
A1 J. ASHMORE
YR 1966
UL http://molpharm.aspetjournals.org/content/2/3/227.abstract
AB Preincubation of rat adipose tissue and hemidiaphragm with nethalide, an adrenergic blocking agent, inhibits the effect of insulin on glucose uptake and 14CO2 production from 14C-labeled glucose. With the use of 131I-labeled insulin, it is possible to demonstrate that blocking of the metabolic effects of insulin is not accompanied by any reduction in 131I bound by the tissue. Addition of guinea pig anti-insulin serum does not affect the proportion of bound radioactive material when rat adipose tissue is incubated with radioinsulin, but markedly reduces insulin binding to rat diaphragm muscle. Incubation of the fat pad with radioinsulin for 90 min reduces the proportion of immunologically active insulin in the medium by about 10%. Experiments described indicate a lack of correlation between the metabolic effects of insulin and the binding of this hormone to tissues.