PT  - JOURNAL ARTICLE
AU  - B. J. GOULD
AU  - P. D. DAWKINS
AU  - M. J. H. SMITH
AU  - A. J. LAWRENCE
TI  - The Mechanism of the Inhibition of Aminotransferases by Salicylate
DP  - 1966 Nov 01
TA  - Molecular Pharmacology
PG  - 526--533
VI  - 2
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/2/6/526.short
4100  - http://molpharm.aspetjournals.org/content/2/6/526.full
SO  - Mol Pharmacol1966 Nov 01; 2
AB  - Salicylate inhibits pig heart alanine and aspartate aminotransferase activities in vitro. The inhibitions are reversible, involving competition with α-oxoglutarate and alanine for the alanine aminotransferase and with α-oxoglutarate and aspartate for the aspartate aminotransferase. The results are consistent with a binary mechanism for the action of the aminotransferases. In addition they show that salicylate inhibits alanine aminotransferase much more strongly than aspartate aminotransferase. These results are discussed with reference to some of the in vivo actions of the drug. ACKNOWLEDGMENT We wish to thank the Arthritis and Rheumatism Research Council for generous financial support and the Nuffield Foundation for a grant towards the purchase of the spectrophotometer used in the work.