PT  - JOURNAL ARTICLE
AU  - SHIGERU YAMABE
TI  - A Spectrophotometric Study on Binding of Acridine Orange with DNA
DP  - 1967 Nov 01
TA  - Molecular Pharmacology
PG  - 556--560
VI  - 3
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/3/6/556.short
4100  - http://molpharm.aspetjournals.org/content/3/6/556.full
SO  - Mol Pharmacol1967 Nov 01; 3
AB  - The interaction of acridine orange (AO) with calf thymus DNA was studied by the ordinary and difference absorption spectra methods. The spectral changes of AO produced with DNA were found to be due to binding of AO with the deoxyadenosine and deoxyguanosine moieties of DNA. It was also found that the contribution of interaction with the deoxycytidine and deoxythymidine moieties to the above spectral changes was less remarkable. A comparative study was made on a typical metachromatic system of AO and heparin. ACKNOWLEDGMENTS The author wishes to thank Dr. M. Nishikawa of Takeda Chemical Industries for kind help in this work. The author wishes also to thank Miss M. Katagiri and Miss K. Tetsubayashi for their technical assistance.