PT  - JOURNAL ARTICLE
AU  - J B Kurz
AU  - J P Perkins
TI  - Isoproterenol-initiated beta-adrenergic receptor diacytosis in cultured cells.
DP  - 1992 Feb 01
TA  - Molecular Pharmacology
PG  - 375--381
VI  - 41
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/41/2/375.short
4100  - http://molpharm.aspetjournals.org/content/41/2/375.full
SO  - Mol Pharmacol1992 Feb 01; 41
AB  - The kinetics of the return of internalized beta-adrenergic receptors to the plasma membrane were measured in human astrocytoma cells. The movement of [125I]iodopindolol-labeled receptors back to the plasma membrane was measured directly and was shown to occur with a t1+2 of 3-4 min. Unlabeled receptors appeared to exhibit the same kinetics of externalization. The process was not inhibited by low concentrations (1-10 microM) of propranolol or even high concentrations of isoproterenol (0.1-1.0 mM). Higher concentrations of propranolol (0.1-1.0 mM) and other lipophilic amines inhibited externalization. The results are consistent with the proposal that catecholamine-induced beta-adrenergic receptor internalization and externalization (diacytosis) occur via the clathrin-coated pit/endosome pathway.