PT  - JOURNAL ARTICLE
AU  - Gudermann, T
AU  - Levy, F O
AU  - Birnbaumer, M
AU  - Birnbaumer, L
AU  - Kaumann, A J
TI  - Human S31 serotonin receptor clone encodes a 5-hydroxytryptamine1E-like serotonin receptor.
DP  - 1993 Mar 01
TA  - Molecular Pharmacology
PG  - 412--418
VI  - 43
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/43/3/412.short
4100  - http://molpharm.aspetjournals.org/content/43/3/412.full
SO  - Mol Pharmacol1993 Mar 01; 43
AB  - We reported recently the molecular cloning of a genomic fragment, designated S31, that has an open reading frame of 1095 nucleotides, encoding a protein of 365 amino acids. Amino acid similarity analysis suggested that the S31 protein could be a guanine nucleotide-binding protein-coupled receptor pertaining to the serotonin receptor subfamily. Expression of the S31 open reading frame in murine L cells confirmed this, because it led to the appearance of serotonin-mediated inhibition of adenylyl cyclase activity, which was absent in the recipient L cells. We now report some aspects of the pharmacological profile of this receptor. We found that the relative potencies with which 5-hydroxytryptamine, 5-carboxamidotryptamine, methysergide, ergotamine, 8-hydroxydipropylaminotetralin, and trifluoromethylphenylpiperazine promote inhibition of adenylyl cyclase are as follows: 5-hydroxytryptamine > methysergide > ergotamine > trifluoromethylphenylpiperazine > or = 8-hydroxydipropylaminotetralin > 5-carboxyamidotryptamine. This corresponds to the rank order of potencies assigned for these drugs for the 1E subtype of serotonin receptors discovered by Leonhardt and collaborators in human brain [J. Neurochem. 53:465-471 (1989)].