PT  - JOURNAL ARTICLE
AU  - L. A. MOUNTER
AU  - R. I. ELLIN
TI  - The Inhibition of Acetylcholinesterase by 2-Pyridinealdoxime Methyl Halide
DP  - 1968 Sep 01
TA  - Molecular Pharmacology
PG  - 452--456
VI  - 4
IP  - 5
4099  - http://molpharm.aspetjournals.org/content/4/5/452.short
4100  - http://molpharm.aspetjournals.org/content/4/5/452.full
SO  - Mol Pharmacol1968 Sep 01; 4
AB  - The effect of varying concentrations of 2-pyridinealdoxime methyl chloride (2-PAM) on the hydrolysis of acetyl-β-methylcholine and acetylcholine by electric eel cholinesterase has been studied. With the former substrate the effects are predominantly competitive, but there are noncompetitive contributions. The dissociation constant of the enzyme-inhibitor complex has been evaluated, together with other kinetic constants. The relative magnitudes of these constants determine the kinetic behavior of the enzymic hydrolysis. Inhibition by 2-PAM chloride and other quaternary compounds may be significant in the interpretation of pharmacological and biochemical experiments.