RT Journal Article
SR Electronic
T1 The Inhibition of Acetylcholinesterase by 2-Pyridinealdoxime Methyl Halide
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 452
OP 456
VO 4
IS 5
A1 L. A. MOUNTER
A1 R. I. ELLIN
YR 1968
UL http://molpharm.aspetjournals.org/content/4/5/452.abstract
AB The effect of varying concentrations of 2-pyridinealdoxime methyl chloride (2-PAM) on the hydrolysis of acetyl-β-methylcholine and acetylcholine by electric eel cholinesterase has been studied. With the former substrate the effects are predominantly competitive, but there are noncompetitive contributions. The dissociation constant of the enzyme-inhibitor complex has been evaluated, together with other kinetic constants. The relative magnitudes of these constants determine the kinetic behavior of the enzymic hydrolysis. Inhibition by 2-PAM chloride and other quaternary compounds may be significant in the interpretation of pharmacological and biochemical experiments.