TY - JOUR T1 - The Inhibition of Acetylcholinesterase by 2-Pyridinealdoxime Methyl Halide JF - Molecular Pharmacology JO - Mol Pharmacol SP - 452 LP - 456 VL - 4 IS - 5 AU - L. A. MOUNTER AU - R. I. ELLIN Y1 - 1968/09/01 UR - http://molpharm.aspetjournals.org/content/4/5/452.abstract N2 - The effect of varying concentrations of 2-pyridinealdoxime methyl chloride (2-PAM) on the hydrolysis of acetyl-β-methylcholine and acetylcholine by electric eel cholinesterase has been studied. With the former substrate the effects are predominantly competitive, but there are noncompetitive contributions. The dissociation constant of the enzyme-inhibitor complex has been evaluated, together with other kinetic constants. The relative magnitudes of these constants determine the kinetic behavior of the enzymic hydrolysis. Inhibition by 2-PAM chloride and other quaternary compounds may be significant in the interpretation of pharmacological and biochemical experiments. ER -