RT Journal Article
SR Electronic
T1 Opioid mu, delta, and kappa receptor-induced activation of phospholipase C-beta 3 and inhibition of adenylyl cyclase is mediated by Gi2 and G(o) in smooth muscle.
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 870
OP 877
VO 50
IS 4
A1 Murthy, K S
A1 Makhlouf, G M
YR 1996
UL http://molpharm.aspetjournals.org/content/50/4/870.abstract
AB In neurons and transformed cell lines, opioid receptors are coupled to various signaling mechanisms involved in Ca2+ mobilization, including inhibition or activation of Ca2+ channels and phospholipase C-beta (PLC-beta), the enzyme responsible for generation of the Ca2+ mobilizing messenger inositol-1,4,5-trisphosphate [Ins(1,4,5)P3]. In the current study, we used selective PLC-beta and G protein antibodies to identify the PLC-beta isozyme activated by opioid receptors in intestinal smooth muscle and the G proteins to which the PLC-beta isozyme and adenylyl cyclase are coupled. [D-Pen2,D-Pen5]Enkephalin, a delta receptor agonist, stimulated Ins(1,4,5)P3 formation, Ca2+ release, and contraction; inhibited forskolin-stimulated cAMP formation in dispersed muscle cells; and stimulated phosphoinositide hydrolysis in plasma membranes; all of the effects were blocked by pertussis toxin. [D-Pen2,D-Pen5]Enkephalin-stimulated Ins(1,4,5)P3 formation, Ca2+ release, and contraction in permeabilized muscle cells and phosphoinositide hydrolysis in plasma membranes were selectively blocked by G beta antibody and PLC-beta 3 antibody; contractions stimulated by [D-Ala2,N-MePhe4,Gly-ol5]enkephalin, a mu receptor agonist, and U-69,593, a kappa receptor agonist, were also blocked by G beta and PLC-beta 3 antibodies. Inhibition of forskolin-stimulated cAMP formation by delta, mu, and kappa receptor agonists was partially blocked by G alpha i2 and G alpha o antibodies and additively blocked by a combination of the antibodies. The delta, mu, and kappa receptor agonists stimulated the binding of guanosine-5'-O-(3-thio)triphosphate to the alpha subunits of Gi2 and G(o) but not to the alpha subunits of other G proteins. We conclude that opioid mu, delta, and kappa receptors are selectively coupled to Gi2 and G(o) in intestinal smooth muscle. The beta gamma subunits of both G proteins activate PLC-beta 3, thereby stimulating Ins(1,4,5)P3-dependent Ca2+ release and smooth muscle contraction, whereas the alpha subunits inhibit adenylyl cyclase activity.