PT  - JOURNAL ARTICLE
AU  - Hiromichi Fujino
AU  - Yoshihisa Kitamura
AU  - Takeshi Yada
AU  - Takashi Uehara
AU  - Yasuyuki Nomura
TI  - Stimulatory Roles of Muscarinic Acetylcholine Receptors on T Cell Antigen Receptor/CD3 Complex-Mediated Interleukin-2 Production in Human Peripheral Blood Lymphocytes
AID  - 10.1124/mol.51.6.1007
DP  - 1997 Jun 01
TA  - Molecular Pharmacology
PG  - 1007--1014
VI  - 51
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/51/6/1007.short
4100  - http://molpharm.aspetjournals.org/content/51/6/1007.full
SO  - Mol Pharmacol1997 Jun 01; 51
AB  - It is known that there are some bidirectional interactions between the nervous and the immune systems via neurotransmitters and cytokines. To clarify whether any neurotransmitters modulate lymphocyte functions, we examined the effects of oxotremorine-M (Oxo-M) on interleukin-2 (IL-2) production in human peripheral blood lymphocytes by using enzyme-linked immunosorbent assays, Northern blot analyses, reverse transcriptase-polymerase chain reaction, and fluorescence-activated cell sorter. Pretreatment of cells with Oxo-M (10 nm to 10 μm) for 4–24 hr enhanced phytohemagglutinin (PHA)-induced IL-2 mRNA expression and markedly increased IL-2 production compared with those induced by PHA alone. Oxo-M alone did not affect IL-2 mRNA expression and IL-2 production. In CD3-positive T cells, pretreatment with Oxo-M for 24 hr enhanced PHA-induced IL-2 production. Furthermore, pretreatment with Oxo-M enhanced PHA-induced mRNA expression of the α and β subunits of IL-2 receptors and DNA synthesis. Cytometric analysis showed Oxo-M treatment did not up-regulate expression of cell surface molecules such as CD3, CD2, CD4, CD8, and IL-2 receptors. These results suggest that activation of muscarinic receptors enhances T cell antigen receptor/CD3-induced IL-2 production.