TY - JOUR T1 - Inhibition of Neuronal Calcium Channels by a Novel Peptide Spider Toxin, DW13.3 JF - Molecular Pharmacology JO - Mol Pharmacol SP - 407 LP - 418 DO - 10.1124/mol.54.2.407 VL - 54 IS - 2 AU - Kathy G. Sutton AU - Chet Siok AU - Anthony Stea AU - Gerald W. Zamponi AU - Steven D. Heck AU - Robert A. Volkmann AU - Michael K. Ahlijanian AU - Terry P. Snutch Y1 - 1998/08/01 UR - http://molpharm.aspetjournals.org/content/54/2/407.abstract N2 - Peptide toxins have proved to be useful agents, both in discriminating between different components of native calcium channel currents and in the molecular isolation and designation of their cloned channel counterparts. Here, we describe the isolation and characterization of the biochemical and physiological properties of a novel 74-amino acid peptide toxin (DW13.3) extracted from the venom of the spiderFilistata hibernalis. The subtype specificity of DW13.3 was investigated using calcium channel currents recorded from two separate expression systems and several different cultured mammalian cell preparations. Overall, DW13.3 potently blocked all native calcium channel currents studied, with the exception of T-type currents recorded from GH3 cells. Examination of transiently expressed calcium channels in oocytes showed that DW13.3 had the highest affinity for α1A, followed by α1B > α1C > α1E. The affinity of DW13.3 for α1B N-type currents varied by 10-fold between expressed channels and native currents. Although block occurred in a similar 1:1 manner for all subtypes, DW13.3 produced a partial block of both α1A currents and P-type currents in cerebellar Purkinje cells. Selective occlusion of the P/Q-type channel ligand ω-conotoxin MVIIC (but not ω-agatoxin IVA) from its binding site in Purkinje neurons suggests that DW13.3 binds to a site close to the pore of the channel. The inhibition of different subtypes of calcium channels by DW13.3 reflects a common “macro” binding site present on all calcium channels except T-type. ER -