TY - JOUR T1 - Structural Analogues of<span class="sc">d</span>-<em>myo</em>-Inositol-1,4,5-trisphosphate and Adenophostin A: Recognition by Cerebellar and Platelet Inositol-1,4,5-trisphosphate Receptors JF - Molecular Pharmacology JO - Mol Pharmacol SP - 741 LP - 748 DO - 10.1124/mol.52.4.741 VL - 52 IS - 4 AU - C. T. Murphy AU - A. M. Riley AU - C. J. Lindley AU - D. J. Jenkins AU - J. Westwick AU - B. V. L. Potter Y1 - 1997/10/01 UR - http://molpharm.aspetjournals.org/content/52/4/741.abstract N2 - Adenophostins A and B, which are metabolic products of the fungusPenicillium brevicompactum, are potent agonists at thed-myo-inositol-1,4,5-trisphosphate [Ins(1,4,5)P3] receptor. In the current study, adenophostin A was ∼50-fold more potent than Ins(1,4,5)P3at both releasing Ca2+ from the intracellular stores of permeabilized platelets and displacing [3H]Ins(1,4,5)P3 from its receptor on rat cerebellar membranes. Various analogues bearing structural features found in the adenophostins and/or Ins(1,4,5)P3 were examined to elucidate the molecular basis for the observed enhanced potency. 2-AMP did not induce Ca2+ release from permeabilized platelets or have any effect on Ins(1,4,5)P3-induced Ca2+ release. Two carbohydrate-based analogues, (2-hydroxyethyl)-α-d-glucopyranoside-2′,3,4-trisphosphate and α,α′-trehalose-3,4,3′,4′-tetrakisphosphate, could induce release of Ca2+ and displace [3H]Ins(1,4,5)P3 from its binding site on rat cerebellar membranes, although both were less potent than Ins(1,4,5)P3. In common with adenophostin A, release of Ca2+ from the intracellular stores could be inhibited by heparin, and both analogues were metabolically resistant. This study is the first to demonstrate the activity of a synthetic disaccharide at the Ins(1,4,5)P3 receptor and that the Ins(1,4,5)P3 receptor is capable of accommodating an increased steric bulk. The minimal importance of the 2-hydroxyl group of Ins(1,4,5)P3 (occupied by the pyranoside oxygen in adenophostin) was confirmed by comparing the activity ofdl-scyllo-Ins(1,2,4)P3 [which differs from Ins(1,4,5)P3 solely by the orientation of this hydroxyl group] with that of Ins(1,4,5)P3. An analogue of this compound, namely,dl-6-CH2OH-scyllo-Ins(1,2,4)P3, which possesses an equatorial hydroxymethyl group analogous to the 5′-hydroxymethyl group of adenophostin, was found to be equipotent to Ins(1,4,5)P3, demonstrating the tolerance of the Ins(1,4,5)P3 receptor to additional steric bulk at this position. ER -