RT Journal Article SR Electronic T1 Modulation of Relative Intrinsic Activity of Agonists at theAlpha-2A Adrenoceptor by Mutation of Residue 351 of G Protein Gi1α JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP 195 OP 201 DO 10.1124/mol.55.2.195 VO 55 IS 2 A1 Vicky N. Jackson A1 Daljit S. Bahia A1 Graeme Milligan YR 1999 UL http://molpharm.aspetjournals.org/content/55/2/195.abstract AB Compared with epinephrine, the relative intrinsic activity of a series of partial agonists to activate fusion proteins between the porcine alpha-2A adrenoceptor and the α-subunit of Gi1 was reduced after a single-point mutation (Cys351Gly) in the G protein. Although UK14304 was close to a full agonist at the fusion construct containing wild-type (Cys351)Gi1α, it was a partial agonist at that containing Gly351Gi1α. Moreover, although clonidine functioned as a good partial agonist to activate the fusion protein containing Cys351Gi1α, it was essentially an antagonist at the Gly351Gi1α-containing fusion protein. By contrast, incorporation of Ile351Gi1α into the fusion protein resulted in all partial agonists displaying higher intrinsic activity relative to epinephrine to activate this fusion protein than the one containing the wild-type G protein sequence.  This is the first demonstration that the relative intrinsic activity of a series of agonists can be modified by a point mutation in a G protein rather than a receptor and indicates that the nature of a key contact site between a G protein and a receptor can selectively regulate partial agonist function. We provide a model for this based on the hydrophobicity of a key receptor-G protein α-subunit interaction interface.