TY - JOUR T1 - Inhibition of Estrogen-Induced Elevation of Cyclic 3',5'-Adenosine Monophosphate in Rat Uterus JF - Molecular Pharmacology JO - Mol Pharmacol SP - 470 LP - 480 VL - 5 IS - 5 AU - CLARA M. SZEGO AU - JUNE S. DAVIS Y1 - 1969/09/01 UR - http://molpharm.aspetjournals.org/content/5/5/470.abstract N2 - The elevation of cyclic 3',5'-AMP which occurs in the uterus of the ovariectomized rat within 30 sec following intravenous administration of estradiol-17β is subject to blockade by immediately prior injection by vein of the beta-adrenergic antagonists propranolol and, to a lesser extent, dichloroisoproterenol. Propranolol exhibited a slight agonistic effect, not hitherto recognized by other criteria; dichloroisoproterenol possessed this capacity to a striking degree, as had been anticipated. Bilateral adrenalectomy virtually abolished the inhibitory effect of dichloroisoproterenol on the response to estradiol-17β while interfering only slightly with that due to propranolol. The alpha-adrenergic blocking agents phentolamine and dibenzyline appeared inert by these criteria. These data constitute the first observations correlating the effects of systemically administered adrenergic blocking agents with the cyclic 3',5'-AMP response to estrogen in time intact uterus, and appear to support the suggestion that "beta-receptor" function may be closely associated with time the adenyl cyclase system. ACKNOWLEDGMENTS The loyal and competent assistance of Kathleen Furamura and Amy Uyematsu in every phase of the glassware preparation is gratefully acknowledged. Thanks are extended to Edgar Kleiner, Richard Huber, and James Previdi for skilled animal care. ER -