@article {Runge-Morris1198, author = {Melissa Runge-Morris and Wei Wu and Thomas A. Kocarek}, title = {Regulation of Rat Hepatic Hydroxysteroid Sulfotransferase (SULT2-40/41) Gene Expression by Glucocorticoids: Evidence for a Dual Mechanism of Transcriptional Control}, volume = {56}, number = {6}, pages = {1198--1206}, year = {1999}, doi = {10.1124/mol.56.6.1198}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Glucocorticoid-inducible hydroxysteroid sulfotransferase (SULT2-40/41) gene transcription was investigated in primary cultured rat hepatocytes transiently transfected with a series of SULT2-40/41 5'-flanking region-luciferase reporter constructs, with emphasis on examining the functional role of an inverted repeat-0 nuclear receptor motif (IR0). Treatment of transfected cultures with any of four glucocorticoids activated luciferase expression from a construct containing 1938 base pairs (bp) of the SULT2-40/41 gene 5'-flanking sequence, whereas deletion of bp -227 to -158 (containing the IR0 motif) largely abolished the effect. On closer analysis, treatment of hepatocyte cultures with either of the potent glucocorticoids dexamethasone [strong cytochrome P-450 3A (CYP3A) inducer] or triamcinolone acetonide (weak CYP3A inducer) produced dose-dependent increases in luciferase activity when hepatocytes were transiently transfected with a construct containing as little as 158 bp of 5'-flanking sequence or containing a mutated IR0 motif. The dexamethasone dose-dependent increase in luciferase activity continued through a dose of 10-6 M when the transfected construct contained the IR0 motif, but was maximal at 10-7 M when the transfected construct lacked the IR0 motif. In contrast, triamcinolone acetonide-induced luciferase activity was maximal at a dose of 10-7 M, irrespective of the presence or absence of the IR0 motif. Coincubation of transfected hepatocytes with 10-8 M dexamethasone and the antiglucocorticoid RU486 inhibited luciferase expression. Luciferase induction by the prototypical CYP3A inducer pregnenolone 16α-carbonitrile was restricted to constructs containing the IR0 motif. These data suggest that glucocorticoid-inducible SULT2-40/41 gene expression occurs through a dual mechanism, whose components possibly involve the glucocorticoid receptor and the pregnane X receptor.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/56/6/1198}, eprint = {https://molpharm.aspetjournals.org/content/56/6/1198.full.pdf}, journal = {Molecular Pharmacology} }