TY - JOUR T1 - Essential Role of Mitogen-Activated Protein Kinase Pathway and c-Jun Induction in Epidermal Growth Factor-Induced Gene Expression of Human 12-Lipoxygenase JF - Molecular Pharmacology JO - Mol Pharmacol SP - 153 LP - 161 VL - 57 IS - 1 AU - Ben-Kuen Chen AU - Hui-Chung Kung AU - Tein-Yi Tsai AU - Wen-Chang Chang Y1 - 2000/01/01 UR - http://molpharm.aspetjournals.org/content/57/1/153.abstract N2 - The role of mitogen-activated protein kinase signaling and the transcription factor c-Jun in epidermal growth factor (EGF)-induced expression of 12-lipoxygenase in human epidermoid carcinoma A431 cells was studied. EGF increased the activation of extracellular signal-regulated kinase (ERK) and c-Jun amino terminal kinase (JNK) in a time-dependent manner. Treatment of the cells with an mitogen-activated protein kinase kinase inhibitor, PD098059 (30 μM), inhibited the EGF- and pSV2ras-induced expression of 12-lipoxygenase mRNA. Transfection of the cells with Ras, ERK2, Rac, JNK dominant negative mutants pMMrasDN, K52R ERK2, RacN17, and mJNK all inhibited the EGF-induced promoter activation of the 12-lipoxygenase gene. EGF induced the expression of c-Jun and the activity of transcription factor activator protein 1 in cells, and these effects were blocked by the treatment with K52R ERK2 and mJNK. Overexpression of c-Jun increased the expression of 12-lipoxygenase mRNA and enzyme activity. Furthermore, the Sp1-binding sites in the promoter region of the 12-lipoxygenase gene were requisite for c-Jun response, which was similar to that previously observed in EGF response. The results indicate that the EGF-induced expression of 12-lipoxygenase in A431 cells was mediated through the Ras-ERK and Ras-Rac-JNK signal pathways. Subsequent induction of c-Jun led by ERK and JNK activation was essential for this EGF response. The American Society for Pharmacology and Experimental Therapeutics ER -