RT Journal Article SR Electronic T1 RNA Editing of the Human Serotonin 5-HT2C Receptor Delays Agonist-Stimulated Calcium Release JF Molecular Pharmacology JO Mol Pharmacol FD American Society for Pharmacology and Experimental Therapeutics SP 859 OP 862 DO 10.1124/mol.58.4.859 VO 58 IS 4 A1 Raymond D. Price A1 Elaine Sanders-Bush YR 2000 UL http://molpharm.aspetjournals.org/content/58/4/859.abstract AB RNA encoding the human 5-HT2C receptor undergoes adenosine-to-inosine RNA editing events at five positions in the putative second intracellular loop, with a corresponding reduction in receptor/G-protein coupling. Agonist-stimulated calcium release was examined in NIH-3T3 fibroblasts stably expressing the nonedited human INI (hINI) or the edited hVSV or hVGV variants. We hypothesized that different receptor isoforms would show altered dynamics of agonist-induced calcium release. The three isoforms showed a rightward shift in agonist concentration-response curves for eliciting calcium release (EC50 values: hINI, 2.2 nM; hVSV, 15 nM; hVGV, 49 nM). Additionally, the hVGV receptor showed a blunted and delayed [Ca2+]i peak compared with the hINI or hVSV receptor isoforms. These distinctions in agonist-induced [Ca2+]i release imply that edited 5-HT2C receptors may produce distinct physiological responses within the central nervous system.