PT  - JOURNAL ARTICLE
AU  - Amrani, Yassine
AU  - Ammit, Alaina J.
AU  - Panettieri, Reynold A.
TI  - Tumor Necrosis Factor Receptor (TNFR) 1, but Not TNFR2, Mediates Tumor Necrosis Factor-α–Induced Interleukin-6 and RANTES in Human Airway Smooth Muscle Cells: Role of p38 and p42/44 Mitogen-Activated Protein Kinases
DP  - 2001 Oct 01
TA  - Molecular Pharmacology
PG  - 646--655
VI  - 60
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/60/4/646.short
4100  - http://molpharm.aspetjournals.org/content/60/4/646.full
SO  - Mol Pharmacol2001 Oct 01; 60
AB  - Little information is available regarding the mechanisms involved in cytokine-induced synthetic function of human airway smooth muscle (ASM) cells. Here, we report that tumor necrosis factor receptor (TNFR) 1-induced p38 and p42/44 mitogen-activated protein kinase (MAPK) activation modulates tumor necrosis factor-α (TNFα)-mediated synthetic responses: expression of intercellular adhesion molecule-1 (ICAM-1) and secretion of interleukin (IL)-6 and the regulated-on-activation, normal T-cell expressed and secreted (RANTES) chemokine in human ASM cells. Pretreatment of ASM cells with SB203580, a p38 MAPK inhibitor, slightly enhanced TNFα-induced ICAM-1 expression in a dose-dependent manner but partially inhibited secretion of RANTES and IL-6. In contrast, PD98059, a p42/44 inhibitor, reduced ICAM-1 expression by 50% but had no effect on TNFα-induced RANTES or IL-6 secretion. SB203580 and PD98059 had little effect on TNFα-induced nuclear factor-κB (NF-κB) activation as determined in cells transfected with a NF-κB–luciferase reporter construct. We also found that agonistic antibodies specific for either TNFR1 or TNFR2 stimulated IL-6 and RANTES secretion and activated p38 and p42/44 MAPKs. In addition, both antibodies induced NF-κB-mediated gene transcription. Using receptor-specific blocking antibodies, we found that TNFR1 primarily regulates TNFα-induced IL-6 and RANTES secretion and activation of p38 and p42/44 MAPK pathways. Interestingly, we found that TNFR1 and TNFR2 are expressed differently on the cell surface of ASM cells. Our data suggest that despite the presence of functional TNFR2, TNFR1 associated with MAPK-dependent and -independent pathways is the primary signaling pathway involved in TNFα-induced synthetic functions in ASM cells. The American Society for Pharmacology and Experimental Therapeutics