TY - JOUR T1 - Activation of Inositol 1,4,5-Trisphosphate Receptor Is Essential for the Opening of Mouse TRP5 Channels JF - Molecular Pharmacology JO - Mol Pharmacol SP - 989 LP - 998 DO - 10.1124/mol.60.5.989 VL - 60 IS - 5 AU - Hideaki Kanki AU - Mariko Kinoshita AU - Akinori Akaike AU - Masamichi Satoh AU - Yasuo Mori AU - Shuji Kaneko Y1 - 2001/11/01 UR - http://molpharm.aspetjournals.org/content/60/5/989.abstract N2 - We studied the opening mechanism of Ca2+-permeable channels formed with mouse transient receptor potential type 5 (mTRP5) usingXenopus oocytes. After stimulation of coexpressed muscarinic M1 receptors with acetylcholine (ACh) in a Ca2+-free solution, switching to 2 mM Ca2+-containing solution evoked a large Cl−current, which reflects the opening of endogenous Ca2+-dependent Cl− channels following Ca2+ entry through the expressed channels. The ACh-evoked response was not affected by a depletion of Ca2+ store with thapsigargin but was inhibited by preinjection of antisense oligodeoxynucleotides (ODNs) to Gq, G11, or both. The mTRP5 channel response was also induced by a direct activation of G proteins with injection of guanosine 5′-3-O-(thio)triphosphate (GTPγS). The ACh- and GTPγS-evoked responses were inhibited by either pretreatment with a phospholipase C inhibitor, U73122, or an inositol-1,4,5-trisphosphate (IP3) receptor inhibitor, xestospongin C (XeC). An activation of IP3 receptors with injection of adenophostin A (AdA) evoked the mTRP5 channel response in a dose-dependent manner. The AdA-evoked response was not suppressed by preinjection of antisense ODNs to Gq/11 or U73122 but was suppressed by either preinjection of XeC or a peptide mimicking the IP3 binding domain of Xenopus IP3 receptor. These findings suggest that the activation of IP3 receptor is essential for the opening of mTRP5 channels, and that neither G proteins, phosphoinositide metabolism, nor depletion of the Ca2+ store directly modifies the IP3receptor-linked opening of mTRP5 channels. ER -