TY - JOUR T1 - Dopamine D<sub>2</sub> Receptor-Induced Heterologous Sensitization of Adenylyl Cyclase Requires Gα<sub>s</sub>: Characterization of Gα<sub>s</sub>-Insensitive Mutants of Adenylyl Cyclase V JF - Molecular Pharmacology JO - Mol Pharmacol SP - 1168 LP - 1172 DO - 10.1124/mol.60.6.1168 VL - 60 IS - 6 AU - Val J. Watts AU - Ronald Taussig AU - Rachael L. Neve AU - Kim A. Neve Y1 - 2001/12/01 UR - http://molpharm.aspetjournals.org/content/60/6/1168.abstract N2 - Whereas acute stimulation of Gαi/o-coupled receptors inhibits the activity of adenylyl cyclase, a delayed consequence of persistent activation of the receptors is heterologous sensitization, an enhanced responsiveness of adenylyl cyclase to activators such as forskolin or agonists of Gαs-coupled receptors. Gαs-insensitive mutants of adenylyl cyclase type V were used to test the hypothesis that heterologous sensitization requires Gαs-dependent activation of adenylyl cyclase. When adenylyl cyclase was stably expressed in human embryonic kidney (HEK) 293 cells with the D2L dopamine receptor, basal, forskolin-stimulated, and isoproterenol-stimulated cyclic AMP accumulation were all enhanced by 2-h pretreatment with the D2 receptor agonist quinpirole. Transient expression of wild-type adenylyl cyclase and three Gαs-insensitive mutants (F379L, R1021Q, and F1093S) in HEK293 cells stably expressing the D2L receptor demonstrated that all three mutants had little or no responsiveness to β-adrenergic receptor-mediated activation of Gαs but that the mutants retained sensitivity to forskolin and to D2L receptor-mediated inhibition. Transiently expressed adenylyl cyclase V was robustly sensitized by 2-h pretreatment with quinpirole. In contrast, the Gαs-insensitive mutants displayed no sensitization of forskolin-stimulated cyclic AMP accumulation, indicating that responsiveness to Gαs is required for the expression of heterologous sensitization. ER -