PT - JOURNAL ARTICLE AU - Reiko Aida AU - Takahiro Moriya AU - Miwa Araki AU - Masashi Akiyama AU - Keiji Wada AU - Etsuko Wada AU - Shigenobu Shibata TI - Gastrin-Releasing Peptide Mediates Photic Entrainable Signals to Dorsal Subsets of Suprachiasmatic Nucleus via Induction of<em>Period</em> Gene in Mice AID - 10.1124/mol.61.1.26 DP - 2002 Jan 01 TA - Molecular Pharmacology PG - 26--34 VI - 61 IP - 1 4099 - http://molpharm.aspetjournals.org/content/61/1/26.short 4100 - http://molpharm.aspetjournals.org/content/61/1/26.full SO - Mol Pharmacol2002 Jan 01; 61 AB - The suprachiasmatic nucleus (SCN), locus of the central circadian clock, consists of two neuronal populations (i.e., a light-recipient ventral SCN subpopulation directly entrained by light and a dorsal SCN subpopulation with an autonomous oscillatory function possessing an indirect or weak light response). However, the mechanism underlying the transmission of photic signals from the ventral to dorsal SCN remains unclear. Because gastrin-releasing peptide (GRP), expressed mainly in the ventral SCN, exerts phase-shifting actions, loss of the GRP receptor intuitively implies a reduction of photic information from the ventral to dorsal SCN. Therefore, using GRP receptor-deficient mice, we examined the involvement of GRP and the GRP receptor in light- and GRP-induced entrainment by the assessment of behavioral rhythm and induction of mousePeriod(mPer) gene in the SCN, which is believed to be a critical for photic entrainment. Administration of GRP during nighttime dose dependently produced a phase delay of behavior in wild-type but not GRP receptor-deficient mice. This phase-shift by GRP was closely associated with induction of mPer1 andmPer2 mRNA as well as c-Fos protein in the dorsal portion of the SCN, where the GRP receptor was also expressed abundantly. Both the light-induced phase shift in behavior and the induction of mPer mRNA and c-Fos protein in the dorsal SCN were attenuated in GRP receptor-deficient mice. Our present studies suggest that GRP neurons in the retinorecipient ventral area of the SCN convey the photic entrainable signals from the ventral SCN to the dorsal SCN via induction of the mPer gene.