RT Journal Article
SR Electronic
T1 Identification of a Novel Dioxin-Inducible Cytochrome P450
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 255
OP 259
DO 10.1124/mol.61.2.255
VO 61
IS 2
A1 Steven P. Rivera
A1 Sirkku T. Saarikoski
A1 Oliver Hankinson
YR 2002
UL http://molpharm.aspetjournals.org/content/61/2/255.abstract
AB Representational difference analysis was used to isolate cDNAs corresponding to 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin)-inducible genes from mouse Hepa-1 cells. One cDNA encoded a novel cytochrome P450. The human homolog was also isolated and later proved to be human CYP2S1. The induction of mouse CYP2S1 mRNA by dioxin represents a primary response and required the aryl hydrocarbon receptor and aryl hydrocarbon receptor nuclear translocator proteins. The induction of CYP2S1 also occurred in mouse liver and lung, with the highest expression found in lung. CYP2S1 was also inducible in a human lung epithelial cell line. The dioxin-inducibility of CY2S1 is exceptional, because all previously well-characterized cases of the induction of cytochromes P450 by dioxin involve members of the CYP1 family.