TY - JOUR T1 - Side-Chain Substitutions within Angiotensin II Reveal Different Requirements for Signaling, Internalization, and Phosphorylation of Type 1A Angiotensin Receptors JF - Molecular Pharmacology JO - Mol Pharmacol SP - 768 LP - 777 DO - 10.1124/mol.61.4.768 VL - 61 IS - 4 AU - Alice C. Holloway AU - Hongwei Qian AU - Luisa Pipolo AU - James Ziogas AU - Shin-ichiro Miura AU - Sadashiva Karnik AU - Bridget R. Southwell AU - Michael J. Lew AU - Walter G. Thomas Y1 - 2002/04/01 UR - http://molpharm.aspetjournals.org/content/61/4/768.abstract N2 - Binding of the peptide hormone angiotensin II (AngII) to the type 1 (AT1A) receptor and the subsequent activation of phospholipase C-mediated signaling, involves specific determinants within the AngII peptide sequence. In contrast, the contribution of such determinants to AT1A receptor internalization, phosphorylation and activation of mitogen-activated protein kinase (MAPK) signaling is not known. In this study, the internalization of an enhanced green fluorescent protein-tagged AT1A receptor (AT1A-EGFP), in response to AngII and a series of substituted analogs, was visualized and quantified using confocal microscopy. AngII-stimulation resulted in a rapid, concentration-dependent internalization of the chimeric receptor, which was prevented by pretreatment with the nonpeptide AT1receptor antagonist EXP3174. Remarkably, AT1A receptor internalization was unaffected by substitution of AngII side chains, including single and double substitutions of Tyr4 and Phe8 that abolish phospholipase C signaling through the receptor. AngII-induced receptor phosphorylation was significantly inhibited by several substitutions at Phe8 as well as alanine replacement of Asp1. The activation of MAPK was only significantly inhibited by substitutions at position eight in the peptide and specific substitutions did not equally inhibit inositol phosphate production, receptor phosphorylation and MAPK activation. These results indicate that separate, yet overlapping, contacts made between the AngII peptide and the AT1A receptor select/induce distinct receptor conformations that preferentially affect particular receptor outcomes. The requirements for AT1A receptor internalization seem to be less stringent than receptor activation and signaling, suggesting an inherent bias toward receptor deactivation. ER -