PT  - JOURNAL ARTICLE
AU  - C. J. PARLI
AU  - G. J. MANNERING
TI  - Induction of Drug Metabolism
DP  - 1970 Mar 01
TA  - Molecular Pharmacology
PG  - 178--183
VI  - 6
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/6/2/178.short
4100  - http://molpharm.aspetjournals.org/content/6/2/178.full
SO  - Mol Pharmacol1970 Mar 01; 6
AB  - Previous studies from this laboratory showed that the administration of 3-methylcholanthrene and 3,4-benzpyrene caused the formation of cytochrome P1-450, which differs from cytochrome P-450 in its substrate specificity, in the difference spectra it produces with ethyl isocyanide, and in its drug-binding properties. Six polycyclic hydrocarbons, previously shown to range in inductive capacities from no activity to very high activity, were administered to rats. The various degrees of induction of 3-methyl-4-methylaminoazobenzene N-demethylase that resulted were related directly to changes in ethyl isocyanide difference spectra and aniline binding difference spectra of microsomal hemoprotein, two measurements which reflect the presence of cytochrome P1-450. When a maximally stimulatory dose of a polycyclic hydrocarbon was given, it caused finite increases in microsomal 3-methyl-4-methylaminoazobenzene N-demethylase activity and cytochrome P1-450 content, whether it was a poor, intermediate, or potent inducing agent. ACKNOWLEDGMENTS The recrystallized polycyclic hydrocarbons were supplied by Dr. J. L. Leong. The authors gratefully acknowledge the able technical assistance of Mrs. Janice Shoeman and Miss Kathy Johnson.