PT  - JOURNAL ARTICLE
AU  - Schuetz, Erin
AU  - Lan, Lubin
AU  - Yasuda, Kazuto
AU  - Kim, Richard
AU  - Kocarek, Thomas A.
AU  - Schuetz, John
AU  - Strom, Stephen
TI  - Development of A Real-Time in Vivo Transcription Assay: Application Reveals Pregnane X Receptor-Mediated Induction of CYP3A4 by Cancer Chemotherapeutic Agents
AID  - 10.1124/mol.62.3.439
DP  - 2002 Sep 01
TA  - Molecular Pharmacology
PG  - 439--445
VI  - 62
IP  - 3
4099  - http://molpharm.aspetjournals.org/content/62/3/439.short
4100  - http://molpharm.aspetjournals.org/content/62/3/439.full
SO  - Mol Pharmacol2002 Sep 01; 62
AB  - We report the development of a rapid real-time assay that measures the transcription of luciferase reporter genes in transduced mouse hepatic cells in vivo. Luciferase activity is noninvasively measured by whole-body optical imaging within hours of the hydrodynamic injection of as little as 1 μg of naked DNA. Transcription of genes introduced as linearized DNA can be serially assayed for weeks in each animal. Transcription was quantified by extracorporal monitoring of bioluminescence as well as or better than by traditional in vitro bioluminescence assay. Our assay allows the measurement of transcription as it occurs, under the most informative biological conditions (i.e., in a living, intact organ). Furthermore, it substantially reduces the cost, time, and number of animals required for analysis of gene expression. The utility of the method is demonstrated in the discovery that topotecan and etoposide are ligands of pregnane X receptor that induce CYP3A4 transcription.