RT Journal Article
SR Electronic
T1 A Na<sup>+</sup>
<strong>/</strong>Cl<sup>−</sup>-Dependent Transporter for Catecholamines, Identified as a Norepinephrine Transporter, Is Expressed in the Brain of the Teleost Fish Medaka (<em>Oryzias latipes</em>)
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 462
OP 473
VO 60
IS 3
A1 Christine Roubert
A1 Corinne Sagné
A1 Marika Kapsimali
A1 Philippe Vernier
A1 Frank Bourrat
A1 Bruno Giros
YR 2001
UL http://molpharm.aspetjournals.org/content/60/3/462.abstract
AB We report the isolation, functional characterization, and localization of a Na+/Cl−-dependent catecholamine transporter (meNET) present in the brain of the teleost fish medaka. This carrier is very similar to the human neuronal norepinephrine transporter (NET) and the human neuronal dopamine transporter (DAT), showing 70 and 64% amino acid identity, respectively. When expressed in COS-7 cells, this transporter mediates the high-affinity uptake of dopamine (KM = 290 nM) and norepinephrine (KM = 640 nM). Its pharmacological profile reveals more similarities with NET, including a high affinity for the tricyclic antidepressants desipramine (IC50 = 0.92 nM) and nortriptyline (IC50 = 16 nM). In situ hybridization on the medaka brain shows that meNET mRNA is present only in a subset of tyrosine hydroxylase-positive neurons found in the noradrenergic areas of the hindbrain, such as the locus ceruleus and area postrema. None of the dopaminergic areas anterior to the isthmus contains any labeled neurons. Neither reverse transcriptase-polymerase chain reaction with degenerate primers specific for γ-aminobutyric acid transporter/NET nor autoradiographic experiments with [125I]3b-(4-iodophenyl)-tropane-2b-carboxylic acid methyl ester revealed an additional catecholamine transporter in the medaka brain. Uptake experiments with medaka brain synaptosomes show an endogenous transport with a pharmacological profile identical to that of the recombinant meNET. Thus, meNET is probably the predominant—if not the only—catecholamine transporter in the medaka fish brain. In view of the highly conserved primary structures and pharmacological properties of meNET, it is tempting to speculate that a specific dopamine transport developed later in vertebrate evolution and probably accompanied the tremendous enlargement of the meso-telencephalic dopaminergic pathways in amniotes. The American Society for Pharmacology and Experimental Therapeutics