RT Journal Article
SR Electronic
T1 Fluorinated Pyrimidines
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 273
OP 280
VO 6
IS 3
A1 YOSHISADA FUJIWARA
A1 TOSHIKAZU OKI
A1 CHARLES HEIDELBERGER
YR 1970
UL http://molpharm.aspetjournals.org/content/6/3/273.abstract
AB 5-Trifluoromethyl-2'-deoxyuridine (F3TdR), a thymidine analogue, is incorporated into the DNA of HeLa and leukemia L5178Y cells in culture. Under conditions of synchrony induced by the production of thymidine deficiency and rescue by thymidine or a thymidine analogue, the addition of F3TdR does not permit any cell division, in contrast to thymidine, 5-bromo-2'-deoxyuridine, or 5-iodo-2'-deoxyuridine, which support cell division. However, F3TdR is incorporated into the cellular DNA, but to only about one-fourth the extent of thymidine. Alkaline sucrose gradient sedimentation revealed that the molecules of DNA containing F3TdR are smaller than normal DNA, suggesting that the rate of growth or joining of chains of DNA is inhibited as a result of the incorporation of the analogue. ACKNOWLEDGMENT We are grateful to Mrs. Barbara Lindsey for skilled technical assistance.