RT Journal Article
SR Electronic
T1 Fluorinated Pyrimidines
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 281
OP 291
VO 6
IS 3
A1 FUJIWARA, YOSHISADA
A1 HEIDELBERGER, CHARLES
YR 1970
UL http://molpharm.aspetjournals.org/content/6/3/281.abstract
AB A comparison has been made of the properties of purified vaccina virions replicated in HeLa cells in the presence of thymidine or the powerfully antiviral 5-trifluoromethyl-2'-deoxyuridine (F3TdR). The replacement of thymidine by the analogue in the DNA of the virions amounted to 1.4-9.8%, and such virions were noninfective. Sucrose density gradient centrifugation of the DNA isolated from purified virions revealed that the normal virions had a sedimentation coefficient to 70, whereas the analogue-containing DNA sedimented with S-values of 52 and 39. The presence of F3TdR in the medium did not affect the process of uncoating of the infecting virions, but recoating of the viral DNA occurred to only 50% of normal. The morphology of vaccinia virions containing F3TdR in their DNA was considerably different from normal.