PT  - JOURNAL ARTICLE
AU  - Kyaw, Moe
AU  - Yoshizumi, Masanori
AU  - Tsuchiya, Koichiro
AU  - Kagami, Shoji
AU  - Izawa, Yuki
AU  - Fujita, Yoshiko
AU  - Ali, Nermin
AU  - Kanematsu, Yasuhisa
AU  - Toida, Kazunori
AU  - Ishimura, Kazunori
AU  - Tamaki, Toshiaki
TI  - Src and Cas Are Essentially but Differentially Involved in Angiotensin II-Stimulated Migration of Vascular Smooth Muscle Cells via Extracellular Signal-Regulated Kinase 1/2 and c-Jun NH<sub>2</sub>-Terminal Kinase Activation
AID  - 10.1124/mol.65.4.832
DP  - 2004 Apr 01
TA  - Molecular Pharmacology
PG  - 832--841
VI  - 65
IP  - 4
4099  - http://molpharm.aspetjournals.org/content/65/4/832.short
4100  - http://molpharm.aspetjournals.org/content/65/4/832.full
SO  - Mol Pharmacol2004 Apr 01; 65
AB  - Angiotensin II (Ang II) plays an important role in several cardiovascular diseases associated with vascular smooth muscle cell (VSMC) growth and migration. Src activity is known to be required for the migration of a number of cell types. p130Cas was reported to be essential for cell migration and actin filament reorganization. Mitogen-activated protein (MAP) kinases were also reported to be critical regulatory factors for growth and migration of VSMC. However, precise intracellular mechanisms involving c-Src, p130Cas, and MAP kinases in Ang II-stimulated migration of VSMC have not been well elucidated. Here we demonstrated that Ang II rapidly and significantly stimulated tyrosine phosphorylation of Src and Cas and their association in rat aortic smooth muscle cells (RASMC). Ang II-stimulated tyrosine phosphorylation of Src and Cas and activation of ERK1/2 and JNK, but not p38, were potently inhibited by Src family tyrosine kinase inhibitors, herbimycin A (HA) and PP2. Ang II-stimulated Src and Cas association, tyrosine phosphorylation of Cas, and activation of ERK1/2 and JNK were suppressed in kinase-inactive Src (KI Src)-overexpressed RASMC. Ang II-stimulated JNK activation but not ERK1/2 activation was blocked in substrate domain-deleted Cas (ΔSD Cas)-overexpressed RASMC. In addition, HA, PP2, ERK1/2 inhibitor, 2′-amino-3′-methoxyflavone (PD98059) and JNK inhibitor, and anthra[1,9-cd]pyrazol-6(2H)-one (SP600125) significantly inhibited Ang II-stimulated migration of RASMC. Ang II-induced colocalization of Src and Cas and migration were inhibited in both KI Src- and ΔSD Cas-overexpressed RASMC. These findings suggest that Src and Cas are essentially but differentially involved in Ang II-stimulated migration of VSMC through the activation of ERK1/2 and JNK.