PT  - JOURNAL ARTICLE
AU  - H. S. Mason
AU  - S. Bourke
AU  - P. J. Kemp
TI  - Selective Modulation of Ligand-Gated P2X Purinoceptor Channels by Acute Hypoxia Is Mediated by Reactive Oxygen Species
AID  - 10.1124/mol.104.000851
DP  - 2004 Dec 01
TA  - Molecular Pharmacology
PG  - 1525--1535
VI  - 66
IP  - 6
4099  - http://molpharm.aspetjournals.org/content/66/6/1525.short
4100  - http://molpharm.aspetjournals.org/content/66/6/1525.full
SO  - Mol Pharmacol2004 Dec 01; 66
AB  - Purinergic excitatory synapses use ATP to mediate fast synaptic transmission via activation of P2X receptor cation channels, and this response can be altered by acute hypoxia. This study examined the effect of acute hypoxia on cloned homo- and heteromeric P2X2 and P2X3 receptors expressed in human embryonic kidney 293 cells. In cells expressing homomeric P2X2 receptors, perfusion of 5 μM ATP (EC25) induced an inward whole-cell current that showed little desensitization during repeated exposures under continuously normoxic conditions. Exposure to a hypoxic ATP solution (pO2, 25-40 mm Hg) significantly reduced the whole-cell current to 49% of normoxic control. This hypoxic inhibition of P2X2-mediated inward current was maintained across all potentials when a voltage-step protocol was applied. In contrast, currents mediated by homomeric P2X3 receptors or heteromeric P2X2/3 receptors were insensitive to an acute hypoxic challenge. One mechanism whereby hypoxia may modulate P2X2 channels is via the production of reactive oxygen species (ROS). H2O2 (1.8 mM) reversibly reduced homomeric P2X2 whole-cell currents to 38% of control. Furthermore, H2O2 attenuated the effect of hypoxia on homomeric P2X2 whole-cell currents. Inhibitors of the mitochondrial electron transport chain that reduce (rotenone and myxothiazol) or increase (antimycin A) the production of ROS altered the magnitude of P2X2-mediated currents. In summary, this is the first report indicating that acute hypoxia is able to regulate the activity of any ligand-gated ion channel. Furthermore, our data show that acute hypoxia selectively modulates the P2X2 receptor and that the response of P2X2 receptor subunits to hypoxia is mediated through the mitochondrial production of ROS.