RT Journal Article
SR Electronic
T1 The Mouse RACK1 Gene Is Regulated by Nuclear Factor-κB and Contributes to Cell Survival
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 1541
OP 1548
DO 10.1124/mol.64.6.1541
VO 64
IS 6
A1 Doo-Sup Choi
A1 Hannah Young
A1 Thomas McMahon
A1 Dan Wang
A1 Robert O. Messing
YR 2003
UL http://molpharm.aspetjournals.org/content/64/6/1541.abstract
AB Receptor for activated C kinase 1 (RACK1) is a multifunctional, WD motif-containing protein important in regulating several cell surface receptors and intracellular protein kinases. To better understand its function, we cloned the mouse RACK1 gene and found it contains eight exons and seven introns, and maps to mouse chromosome 11B1.2-1.3. Promoter analysis identified NF-κB as an important transcription factor for promoter activity. In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-κB (NF-κB), maintained RACK1 levels and promoted cell survival in serum-free medium. Inhibitors of NF-κB activation blocked NGF-stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF. Thus, RACK1 gene expression is induced by NF-κB and RACK1 contributes to NF-κB-mediated cell survival.