@article {McGrew252, author = {Lori McGrew and Raymond D. Price and Elizabeth Hackler and Mike S. S. Chang and Elaine Sanders-Bush}, title = {RNA Editing of the Human Serotonin 5-HT2C Receptor Disrupts Transactivation of the Small G-Protein RhoA}, volume = {65}, number = {1}, pages = {252--256}, year = {2004}, doi = {10.1124/mol.65.1.252}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {The human serotonin 5-HT2C receptor undergoes adenosineto-inosine RNA editing at five positions, generating multiple receptor isoforms with altered G-protein coupling properties. In the current study, we demonstrate that RNA editing regulates the pattern of intracellular signaling. The non-edited human 5-HT2C receptor isoform INI activates phospholipase D via the G13 heterotrimer G-protein. We present evidence that transactivation of the small G-protein RhoA is required for phospholipase D activation. In contrast, neither transactivation of RhoA nor phospholipase D activation was detected in cells expressing the fully edited VGV isoform. The ability to activate phospholipase C is also reduced in VGV-expressing cells, but not to the extent found for the phospholipase D signal. We conclude that RNA editing represents a novel mechanism for regulating 5-HT2C receptor signaling to pathways linked to actin cytoskeletal organization and regulated exocytosis.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/65/1/252}, eprint = {https://molpharm.aspetjournals.org/content/65/1/252.full.pdf}, journal = {Molecular Pharmacology} }