RT Journal Article
SR Electronic
T1 Organotin Compounds Promote Adipocyte Differentiation as Agonists of the Peroxisome Proliferator-Activated Receptor γ/Retinoid X Receptor Pathway
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 766
OP 774
DO 10.1124/mol.104.008409
VO 67
IS 3
A1 Tomohiko Kanayama
A1 Naoki Kobayashi
A1 Satoru Mamiya
A1 Tsuyoshi Nakanishi
A1 Jun-ichi Nishikawa
YR 2005
UL http://molpharm.aspetjournals.org/content/67/3/766.abstract
AB Nuclear receptors play important roles in the maintenance of the endocrine system, regulation of organ differentiation, and fetal development. Endocrine disruptors exert their adverse effects by disrupting the endocrine system via various mechanisms. To assess the effects of endocrine disruptors on nuclear receptors, we developed a high-throughput method for identifying activators of nuclear receptors. Using this system, we found that triphenyltin and tributyltin were activators of peroxisome proliferator-activated receptor (PPAR) γ and retinoid X receptor. Because PPARγ is a master regulator of adipocyte differentiation, we assessed the effect of organotin compounds on preadipocyte 3T3-L1 cells. We found that organotin compounds stimulated differentiation of 3T3-L1 cells as well as expression of adipocyte marker genes.