PT  - JOURNAL ARTICLE
AU  - Martin Gassmann
AU  - Corinne Haller
AU  - Yanick Stoll
AU  - Said Abdel Aziz
AU  - Barbara Biermann
AU  - Johannes Mosbacher
AU  - Klemens Kaupmann
AU  - Bernhard Bettler
TI  - The RXR-Type Endoplasmic Reticulum-Retention/Retrieval Signal of GABA<sub>B1</sub> Requires Distant Spacing from the Membrane to Function
AID  - 10.1124/mol.104.010256
DP  - 2005 Jul 01
TA  - Molecular Pharmacology
PG  - 137--144
VI  - 68
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/68/1/137.short
4100  - http://molpharm.aspetjournals.org/content/68/1/137.full
SO  - Mol Pharmacol2005 Jul 01; 68
AB  - Functional γ-aminobutyric acid type B (GABAB) receptors are normally only observed upon coexpression of GABAB1 with GABAB2 subunits. A C-terminal arginine-based endoplasmic reticulum (ER) retention/retrieval signal, RSRR, prevents escape of unassembled GABAB1 subunits from the ER and restricts surface expression to correctly assembled heteromeric receptors. The RSRR signal in GABAB1 is proposed to be shielded by C-terminal coiled-coil interaction of the GABAB1 with the GABAB2 subunit. Here, we investigated whether the RSRR motif in GABAB1 remains functional when grafted to ectopic sites. We found that the RSRR signal in GABAB1 is inactive in any of the three intracellular loops but remains functional when moved within the distal zone of the C-terminal tail. C-terminal deletions that position the RSRR signal closer to the plasma membrane drastically reduce its effectiveness, supporting that proximity to the membrane restricts access to the RSRR motif. Functional ectopic RSRR signals in GABAB1 are efficiently inactivated by the GABAB2 subunit in the absence of coiled-coil dimerization, supporting that coiled-coil interaction is not critical for release of the receptor complex from the ER. The data are consistent with a model in which removal of RSRR from its active zone rather than its direct shielding by coiled-coil dimerization triggers forward trafficking. Because arginine-based intracellular retention signals of the type RXR, where X represents any amino acid, are used to regulate assembly and surface transport of several multimeric complexes, such a mechanism may apply to other proteins as well.