PT  - JOURNAL ARTICLE
AU  - Zakaria Mtchedlishvili
AU  - Jaideep Kapur
TI  - High-Affinity, Slowly Desensitizing GABA<sub>A</sub> Receptors Mediate Tonic Inhibition in Hippocampal Dentate Granule Cells
AID  - 10.1124/mol.105.016683
DP  - 2006 Feb 01
TA  - Molecular Pharmacology
PG  - 564--575
VI  - 69
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/69/2/564.short
4100  - http://molpharm.aspetjournals.org/content/69/2/564.full
SO  - Mol Pharmacol2006 Feb 01; 69
AB  - The tonic form of GABA-mediated inhibition requires the presence of slowly desensitizing GABAA receptors with high affinity, which has not yet been directly demonstrated in hippocampal neurons. Low concentration of GABA (1 μM) persistently increased baseline noise, increased membrane slope conductance, but did not affect spontaneous inhibitory postsynaptic currents (sIPSCs) in dentate granule cells (DGCs). Higher concentrations of GABA (10–100 μM) desensitized synaptic currents quickly, and there was a large residual current. Saturating concentration of GABA (1 mM) completely desensitized synaptic currents and revealed a slowly desensitizing, persistent current. Penicillin (300 μM) inhibited baseline noise without affecting mean current and inhibited decay time of sIPSCs. GABAA receptors mediating baseline noise in DGCs were sensitive to allopregnanolone, furosemide, and loreclezole and insensitive to diazepam and zolpidem. These studies demonstrate persistently open GABAA receptors on DGCs with high affinity for GABA, slow desensitization rate, and pharmacological properties similar to those of recombinant receptors containing α4, β1, and the δ subunits.