TY - JOUR T1 - Evidence for a Medium-Chain Fatty Acid:Coenzyme A Ligase (Adenosine Monophosphate) That Activates Salicylate JF - Molecular Pharmacology JO - Mol Pharmacol SP - 260 LP - 268 VL - 7 IS - 3 AU - PAUL G. KILLENBERG AU - EUGENE D. DAVIDSON AU - LESLIE T. WEBSTER, JR. Y1 - 1971/05/01 UR - http://molpharm.aspetjournals.org/content/7/3/260.abstract N2 - A medium-chain fatty acid:CoA ligase (AMP) which activates salicylate has been purified approximately 30-fold from bovine liver "mitochondria." The new enzyme is not completely free of a medium-chain fatty acid:CoA ligase (AMP) (EC 6.2.1.2) of the type first described by Mahler, Wakil, and Bock [J. Biol. Chem. 204, 453 (1953)], but the two enzymes can be distinguished readily from each other. The enzyme with salicyl-CoA synthetase activity is more easily inactivated, migrates more slowly toward the anode during disc gel electrophoresis, and utilizes a wider spectrum of aromatic acid substrates than the enzyme described earlier. Evidence was obtained that both enzymes could catalyze the activation of hexanoate, benzoate, o-methoxybenzoate, and anthranilate, but only the "salicylate" enzyme displayed activity with salicylate and p-aminosalicylate. ACKNOWLEDGMENT The authors wish to thank Dr. Sung Ling Yuan for his valuable technical assistance. ER -