@article {MITANI280, author = {F. MITANI and A. P. ALVARES and S. SASSA and A. KAPPAS}, title = {Preparation and Properties of a Solubilized Form of Cytochrome P-450 from Chick Embryo Liver Microsomes}, volume = {7}, number = {3}, pages = {280--292}, year = {1971}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {A procedure for the partial purification and subsequent solubilization of cytochrome P-450 from microsomal membranes of chick embryo liver is described. Successive treatment of microsomes with Protease VII and Lubrol WX yielded a solubilized preparation with about a 1.8-fold increase in the specific content of cytochrome P-450, measured as millimicromoles per milligram of protein, with no detectable amounts of cytochrome b5 and minimal activities of NADPH- and NADH-cytochrome c reductases. The CO difference spectrum of the solubilized cytochrome P-450 showed an absence of cytochrome P-420; however, the absolute spectrum of the CO complex of the reduced preparation displayed a peak at 420 m{\textmu}. The solubilized cytochrome P-450 obtained from chick embryo livers interacted with various substrates to give type I or type II spectra similar to those observed in other species. Cytochrome P-450 in chick embryo liver was inducible by drugs, polycyclic hydrocarbons, and steroids.}, issn = {0026-895X}, URL = {https://molpharm.aspetjournals.org/content/7/3/280}, eprint = {https://molpharm.aspetjournals.org/content/7/3/280.full.pdf}, journal = {Molecular Pharmacology} }