RT Journal Article
SR Electronic
T1 Nitric Oxide Donor, (±)-<em>S</em>-Nitroso-<em>N</em>-acetylpenicillamine, Stabilizes Transactive Hypoxia-Inducible Factor-1α by Inhibiting von Hippel-Lindau Recruitment and Asparagine Hydroxylation
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 236
OP 245
DO 10.1124/mol.108.045278
VO 74
IS 1
A1 Young-Kwon Park
A1 Dae-Ro Ahn
A1 Myoungsuk Oh
A1 Taekyoung Lee
A1 Eun Gyeong Yang
A1 Miwon Son
A1 Hyunsung Park
YR 2008
UL http://molpharm.aspetjournals.org/content/74/1/236.abstract
AB We have confirmed that the NO donor (±)-S-nitroso-N-acetylpenicillamine (SNAP) stabilizes the transactive form of hypoxia-inducible factor-1α (HIF-1α), leading to the induction of HIF-1α target genes such as vascular endothelial growth factor and carbonic anhydrase 9. Activation of HIF-1α should require inhibition of the dual system that keeps it inactive. One is ubiquitination, which is triggered by hydroxylation of HIF-1α-proline and the subsequent binding of E3 ubiquitin ligase, the von Hippel Lindau (VHL) protein. The other is hydroxylation of HIF-1α-asparagine, which reduces the affinity of HIF-1α for its coactivator, cAMP responsive element binding protein/p300. We examined the effects of the NO donor SNAP on proline and asparagine hydroxylation of HIF-1α peptides by measuring the activities of the corresponding enzymes, HIF-1α-specific proline hydroxylase 2 (PHD2) and the HIF-1α-specific asparagine hydroxylase, designated factor inhibiting HIF-1α (FIH-1), respectively. We found that the SNAP did not prevent PHD2 from hydroxylating the proline of HIF-1α. Instead, it blocked the interaction between VHL and the proline-hydroxylated HIF-1α, but only when the reducing agents Fe(II) and vitamin C were limiting. The fact that the absence of cysteine 520 of HIF-1α abolishes its responsiveness to SNAP suggests that this residue mediates the inhibition by SNAP of the interaction between VHL and HIF-1α, presumably by S-nitrosylation of HIF-1α. Un-like PHD2, asparagine hydroxylation by FIH-1 was directly inhibited by SNAP, but again only when reducing agents were limiting. Substitution of cysteine 800 of HIF-1α with alanine failed to reverse the inhibitory effects of SNAP on asparagine hydroxylation, implying that FIH-1, not its substrate HIF-1α, is inhibited by SNAP. The American Society for Pharmacology and Experimental Therapeutics