TY - JOUR T1 - Chromatography of Radioactive Microsomal Hemoproteins on Diethylaminoethyl Cellulose JF - Molecular Pharmacology JO - Mol Pharmacol SP - 500 LP - 510 VL - 7 IS - 5 AU - W. LEVIN AU - R. KUNTZMAN AU - A. ALVARES AU - A. H. CONNEY Y1 - 1971/09/01 UR - http://molpharm.aspetjournals.org/content/7/5/500.abstract N2 - Administration of 14C- or 3H-δ-aminolevulinic acid to immature male rats resulted in the incorporation of radioactivity into liver microsomal hemoprotein. Column chromatography of "solubilized" microsomes or CO-binding particles4 obtained from rats treated with phenobarbital or 3-methylcholanthrene revealed that essentially all the radioactivity was associated with fractions containing cytochromes b5, P-450, P-448, and P-420. The results indicated that radioactivity incorporated into microsomes and CO-binding particles after administration of 14C- or 3H-δ-aminolevulinic acid is associated almost exclusively with cytochromes, and that very little, if any, nonspecific binding of heme occurs. ACKNOWLEDGMENT The authors wish to thank Mrs. P. Althoff for typing the manuscript. ER -