PT - JOURNAL ARTICLE AU - F. MITANI AU - R. J. WINCHESTER AU - A. P. ALVARES AU - A. P. POLAND AU - A. KAPPAS TI - Interaction of Cytochrome P-450 with Antibodies Raised against a Solubilized P-450 Preparation from Hepatic Microsomes DP - 1971 Nov 01 TA - Molecular Pharmacology PG - 689--696 VI - 7 IP - 6 4099 - http://molpharm.aspetjournals.org/content/7/6/689.short 4100 - http://molpharm.aspetjournals.org/content/7/6/689.full SO - Mol Pharmacol1971 Nov 01; 7 AB - Soluble cytochrome P-450 prepared from microsomes of rat liver or from livers of chick embryos was shown to be capable of eliciting an immunological response in rabbits. The γ-globulin fraction from sera of immunized rabbits reacted with soluble P-450 of the same species. Precipitating reactions were obtained when antibody and antigen were incubated in the fluid phase, as well as by agar diffusion techniques. Lack of cross-reactivity between species was demonstrated when antibody to rat cytochrome P-450 was allowed to react with soluble P-450 from chick embryo livers and when antibody to chick cytochrome P-450 was allowed to react with soluble P-450 from rat liver. The Precipitate obtained upon reaction of antibody and antigen from the same species showed a typical CO difference spectrum with an absorption maximum at 450 nm and a type II difference spectrum with aniline. However, no type I difference spectrum could be obtained after the addition of sodium hexobarbital to the antibody-antigen complex, suggesting that the binding site of the immune complex may be at or near the type I binding site.