RT Journal Article
SR Electronic
T1 The Metabolism of Aminopyrine in Chick Embryo Hepatic Cell Culture: Effects of Competitive Substrates and Carbon Monoxide
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 697
OP 705
VO 7
IS 6
A1 POLAND, ALAN
A1 KAPPAS, ATTALLAH
YR 1971
UL http://molpharm.aspetjournals.org/content/7/6/697.abstract
AB Chick embryonic liver cells grown in monolayer cultures metabolize aminopyrine to 4-aminoantipyrine, which is released into the medium. This N-demethylation is performed by liver cell cultures but not by renal or intestinal cell cultures from chick embryos. The formation of 4-aminoantipyrine is linear with time and is inhibited by SKF 525-A (2-diethylaminoethyl 2,2-diphenylvalerate HCl), piperonyl butoxide, hexobarbital, lauric acid, and testosterone. Inhibition in all cases is at least partially reversible. The rate of 4-aminoantipyrine formation is also reversibly inhibited by carbon monoxide. From the CO:O2 ratio and rate of formation of the metabolite, it is possible to estimate the partition coefficient, KL, for cytochrome P-450 in living cells, which agrees quite well with estimates from incubations in vitro. ACKNOWLEDGMENT We would like to thank Mrs. Sandra Weinstein for excellent technical assistance.