PT  - JOURNAL ARTICLE
AU  - VINCENT E. REID
AU  - MORRIS FRIEDKIN
TI  - Thymidylate Synthetase in Mouse Erythrocytes Infected with <em>Plasmodium berghei</em>
DP  - 1973 Jan 01
TA  - Molecular Pharmacology
PG  - 74--80
VI  - 9
IP  - 1
4099  - http://molpharm.aspetjournals.org/content/9/1/74.short
4100  - http://molpharm.aspetjournals.org/content/9/1/74.full
SO  - Mol Pharmacol1973 Jan 01; 9
AB  - Thymidylate synthetase in mouse erythrocytes infected with Plasmodium berghei increases markedly during cellular parasitization. The malaria enzyme has been compared with enzymes from normal mouse reticulocytes and Escherichia coli by kinetic and physical means. When folic acid analogues were used as cofactors, distinct kinetic differences were apparent when thymidylate synthetases from P. berghei and E. coli were compared. However, no significant kinetic dissimilarities were revealed in a comparison of enzymes from P. berghei and the host mouse reticulocytes. Upon gel filtration the P. berghei thymidylate synthetase exhibits an apparent molecular weight in excess of 100,000 whereas the normal mouse reticulocyte enzyme shows an apparent molecular weight of 68,000, similar to the enzyme from E. coli.