RT Journal Article
SR Electronic
T1 Stimulation by Acetylcholine of Sulfated Mucopolysaccharide Release from the Perfused Cat Adrenal Gland
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 590
OP 594
VO 9
IS 4
A1 R. K. MARGOLIS
A1 S. D. JAANUS
A1 R. U. MAUGOLIS
YR 1973
UL http://molpharm.aspetjournals.org/content/9/4/590.abstract
AB Sulfated complex carbohydrates in the adrenal medulla were labeled with 35SO4- after the administration of insulin to deplete catecholamine stores. Twenty-four hours later the left adrenal gland was perfused in situ with Locke's solution. Addition of acetylcholine to the perfusion solution resulted in a 12-20-fold increase in the release of sulfate-labeled nondialyzable material, together with the usual large increase in catecholamine secretion. Digestion of the sulfate-labeled macromolecular material with bacterial chondroitinases demonstrated that 65-80% of the radioactivity was in the form of chondroitin 4- and 6-sulfate. The remaining radioactivity was present in an N-sulfated mucopolysaccharide which was tentatively identified as heparan sulfate. The results of these experiments are consistent with our previous finding of sulfated mucopolysaccharides, consisting mostly of chondroitin 4- and 6-sulfate, in purified chromaffin granules from bovine adrenal medulla, and are discussed in terms of the possible role of anionic complex carbohydrates in the storage and release of biogenic amines.