PT  - JOURNAL ARTICLE
AU  - ENRIQUE OCHOA
AU  - SARA FISZER DE PLAZAS
AU  - EDUARDO DE ROBERTIS
TI  - Conductance Changes Produced by <em>l</em>-Norepinephrine on Lipid Membranes Containing a Proteolipid from the Bovine Spleen Capsule
DP  - 1972 Mar 01
TA  - Molecular Pharmacology
PG  - 215--221
VI  - 8
IP  - 2
4099  - http://molpharm.aspetjournals.org/content/8/2/215.short
4100  - http://molpharm.aspetjournals.org/content/8/2/215.full
SO  - Mol Pharmacol1972 Mar 01; 8
AB  - A proteolipid isolated from bovine spleen capsule, with a high affinity for binding [7-3H]dl-norepinephrine, has been incorporated into artificial lipid bilayer membranes that separate two aqueous phases containing ions. To one of these phases several drugs were added, and the electrical conductance changes were recorded. The addition of l-norepinephrine produced a transient conductance change whose amplitude was related to the dose. l-Isoproterenol gave a smaller response, and d-norepinephrine had no effect. Membranes made with large amounts of phosphatidylcholine also reacted to l-norepinephrine. Since this phospholipid is absent from the proteolipid which binds norepinephrine, it is conucluded that phosphatidylcholine gives a nonspecific reaction. Several control experiments and the observation that the conductance change toward l-norepinepinephrine is blocked with phentolamine suggest that this proteolipid may play a receptor role in the spleen capsule. ACKNOWLEDGMENTS The authors thank Dr. M. Parisi for his helpful suggestions on the techniques for making artificial membranes, and Dr. F. Luduefia of Sterling-Winthrop for providing l-norepinephrine and l-isoproterenol.