RT Journal Article
SR Electronic
T1 Binding of Mazindol and Analogs to the Human Serotonin and Dopamine Transporters
JF Molecular Pharmacology
JO Mol Pharmacol
FD American Society for Pharmacology and Experimental Therapeutics
SP 208
OP 217
DO 10.1124/mol.113.088922
VO 85
IS 2
A1 Kasper Severinsen
A1 Heidi Koldsø
A1 Katrine Almind Vinberg Thorup
A1 Christina Schjøth-Eskesen
A1 Pernille Thornild Møller
A1 Ove Wiborg
A1 Henrik Helligsø Jensen
A1 Steffen Sinning
A1 Birgit Schiøtt
YR 2014
UL http://molpharm.aspetjournals.org/content/85/2/208.abstract
AB Mazindol has been explored as a possible agent in cocaine addiction pharmacotherapy. The tetracyclic compound inhibits both the dopamine transporter and the serotonin transporter, and simple chemical modifications considerably alter target selectivity. Mazindol, therefore, is an attractive scaffold for both understanding the molecular determinants of serotonin/dopamine transporter selectivity and for the development of novel drug abuse treatments. Using molecular modeling and pharmacologic profiling of rationally chosen serotonin and dopamine transporter mutants with respect to a series of mazindol analogs has allowed us to determine the orientation of mazindol within the central binding site. We find that mazindol binds in the central substrate binding site, and that the transporter selectivity can be modulated through mutations of a few residues in the binding pocket. Mazindol is most likely to bind as the R-enantiomer. Tyrosines 95 and 175 in the human serotonin transporter and the corresponding phenylalanines 75 and 155 in the human dopamine transporter are the primary determinants of mazindol selectivity. Manipulating the interaction of substituents on the 7-position with the human serotonin transporter Tyr175 versus dopamine transporter Phe155 is found to be a strong tool in tuning the selectivity of mazindol analogs and may be used in future drug design of cocaine abuse pharmacotherapies.