PT  - JOURNAL ARTICLE
AU  - Xinliang Mao
AU  - Tingjun Hou
AU  - Biyin Cao
AU  - Wenjie Wang
AU  - Zhihua Li
AU  - Suning Chen
AU  - Min Fei
AU  - Rose Hurren
AU  - Marcela Gronda
AU  - Depei Wu
AU  - Suzzane Trudel
AU  - Aaron D Schimmer
TI  - The tricyclic anti-depressant amitriptyline inhibits D-cyclin transactivation and induces myeloma cell apoptosis by inhibiting histone deacetylases:<em>in vitro </em> and <em> in silico </em> evidence
AID  - 10.1124/mol.110.068122
DP  - 2011 Jan 10
TA  - Molecular Pharmacology
PG  - mol.110.068122
4099  - http://molpharm.aspetjournals.org/content/early/2011/01/10/mol.110.068122.short
4100  - http://molpharm.aspetjournals.org/content/early/2011/01/10/mol.110.068122.full
AB  - Amitriptyline is a classic tricyclic antidepressant (TCA) and has been used to treat depression and anxiety of cancer patients but its relevance to cancer cell apoptosis is not known. In the present study, we demonstrated that amitriptyline inhibited cyclin D2 transactivation and displayed potential anti-myeloma activity by inhibiting histone deacetylases (HDACs). Amitriptyline markedly decreased cyclin D2 promoter-driven luciferase activity, reduced cyclin D2 expression and arrested cells at the G0/G1 phase of the cell cycle. Amitriptyline-induced apoptosis was confirmed by Annexin V staining, cleavage of caspase-3 and PARP-1. D-cyclin expression is reported to be epigenetically regulated by histone acetylation. Thus, we examined the effects of amitriptyline on Histone (H3) acetylation and demonstrated that amitriptyline increased acetylation of H3 and expression of p27 and p21. Further studies indicated that amitriptyline downregulated at least HDAC3, -6, -7 and -8, but not HDAC2 in examined myeloma cell lines. Molecular docking analysis and molecular dynamics simulations revealed that amitriptyline bound to HDAC7 and formed strong van der Waals interactions with five residues of HDAC7, including Phe162, His192, Phe221, Leu293 and His326, thus inhibiting HDAC activity. Therefore, we found that amitriptyline inhibited cyclin D2 transactivation and HDAC activity and could be a promising treatment for multiple myeloma.