TY - JOUR T1 - TCL1A SNPs and estrogen-mediated toll-like receptor-MYD88-dependent NF-κB activation: SNP and SERM-dependent modification of inflammation and immune response JF - Molecular Pharmacology JO - Mol Pharmacol DO - 10.1124/mol.117.108340 SP - mol.117.108340 AU - Ming-Fen Ho AU - James N Ingle AU - Tim Bongartz AU - Krishna R Kalari AU - Paul E Goss AU - Lois E Shepherd AU - Taisei Mushiroda AU - Michiaki Kubo AU - Liewei Wang AU - Richard M Weinshilboum Y1 - 2017/01/01 UR - http://molpharm.aspetjournals.org/content/early/2017/06/13/mol.117.108340.abstract N2 - In a previous genome-wide association study (GWAS) for musculoskeletal adverse events during aromatase inhibitor therapy of breast cancer, we reported that single nucleotide polymorphisms (SNPs) near the TCL1A gene were associated with this adverse drug reaction. Functional genomic studies showed that TCL1A expression was induced by estradiol (E2), but only in cells with the variant sequence for the top GWAS SNP (rs11849538), a SNP that created a functional estrogen response element. In addition, TCL1A genotype influenced the "downstream" expression of a series of cytokines and chemokines and had a striking effect on NF-κB transcriptional activity. Furthermore, this SNP-dependent regulation could be "reversed" by selective estrogen receptor modulators (SERMs). The present study was designed to pursue mechanisms underlying TCL1A SNP-mediated, estrogen-dependent NF-κB activation. Functional genomic studies were performed with a panel of 300 lymphoblastoid cell lines for which we had generated genome-wide SNP and gene expression data. It is known that toll-like receptors (TLRs) can regulate NF-κB signaling by a process that requires the adaptor protein MYD88. We found that TLR2, TLR7, TLR9 and TLR10 expression, as well as that of MYD88, could be modulated by TCL1A in a SNP and estrogen-dependent fashion and that these effects were reversed in the presence of SERMs. Furthermore, MYD88 inhibition blocked the TCL1A SNP and estrogen-dependent NF-κB activation as well as protein-protein interaction between TCL1A and MYD88. These observations greatly expand the range of pathways influenced by TCL1A genotype and raise the possibility that this estrogen and SNP-dependent regulation might be altered pharmacologically by SERMs. ER -