Residues in bovine rhodopsin found in SASA calculations to be protected by retinal and aligned residues of representative receptors that have been implicated in ligand binding.
| Residues | SASA % | Representative Receptors | References | ||
|---|---|---|---|---|---|
| NR | WR | Δ | |||
| Glu1133.28 | 0.4 | 0.0 | 0.4 | D2DR, ACM1, NK2R | Bhogal et al., 1994; Matsui et al., 1995; Simpson et al., 1999 |
| Gly1143.29 | 0.3 | 0.0 | 0.3 | D2DR, P2YR, GASR, MSHR, ACTR | Kopin et al., 1995; Naville et al., 1996; Jiang et al., 1997;Yang et al., 1997; Simpson et al., 1999 |
| Ala1173.32 | 13.1 | 2.4 | 10.7 | 5H1A, B2AR, A2AA, D2DR, HH2R, ACM1, ETBR | Strader et al., 1987, 1988, 1991; Fraser et al., 1989; Wang et al., 1991; Gantz et al., 1992; Ho et al., 1992;Mansour et al., 1992; Zhu et al., 1992 |
| Thr1183.33 | 15.1 | 0.0 | 15.1 | ACM3, AA1R | Wess et al., 1991; Rivkees et al., 1999; |
| Gly1213.36 | 6.1 | 0.0 | 6.1 | 5H2A | Almaula et al., 1996 |
| Glu1223.37 | 10.7 | 0.0 | 10.7 | AA2A, AA1R, P2YR | Jiang et al., 1996; Moro et al., 1998; Rivkees et al., 1999 |
| Met2075.42 | 10.6 | 0.3 | 10.3 | 5H1A, A2AA, B2AR | Wang et al., 1991; Ho et al., 1992; Liapakis et al., 2000 |
| Phe2085.43 | 7.3 | 5.0 | 2.3 | D2DR, B2AR | Strader et al., 1989; Mansour et al., 1992 |
| His2115.46 | 1.6 | 0.5 | 1.1 | A2AA, D2DR, B2AR | Strader et al., 1989; Wang et al., 1991; Mansour et al., 1992 |
| Phe2125.47 | 21.6 | 14.6 | 7.0 | A1AA, 5H2A, D2DR | Cho et al., 1995; Wetzel et al., 1996; Shapiro et al., 2000 |
| Phe2616.44 | 5.1 | 0.9 | 4.2 | ET1R | Breu et al., 1995 |
| Trp2656.48 | 13.0 | 0.5 | 12.5 | 5H1B, 5H2A | Roth et al., 1997; Granas and Larhammar, 1999; |
| Tyr2686.51 | 14.0 | 0.0 | 14.0 | A1AB | Chen et al., 1999 |
| Ala2696.52 | 14.8 | 3.1 | 11.7 | 5H1B, 5H2A, B2AR | Dixon et al., 1988; Choudhary et al., 1993; Granas et al., 1998 |
| Ala2726.55 | 1.0 | 0.3 | 0.7 | B2AR, D2DR | Woodward et al., 1994; Wieland et al., 1996 |
| Ala2927.39 | 5.5 | 0.0 | 5.5 | 5H1A, 5H1B, B2AR, A2AA, ACM1, P2UR, GASR, P2YR | Suryanarayana et al., 1991; Guan et al., 1992; Oksenberg et al., 1992; Suryanarayana and Kobilka, 1993; Erb et al., 1995; Kopin et al., 1995; Matsui et al., 1995; Jiang et al., 1997 |
| Ala2957.42 3-a | 2.6 | 0.0 | 2.6 | ACM1, AA1R, AG2R, NTR1 | Savarese et al., 1992; Tucker et al., 1994; Perlman et al., 1997; Labbe-Jullie et al., 1998 |
| Lys2967.43 | 3.3 | 0.8 | 2.5 | 5H2A, AA2A, AA1R, P2YR, PAFR | Olah et al., 1992; Kim et al., 1995; Ishii et al., 1997; Jiang et al., 1997; Roth et al., 1997 |
Data are shown as the percentage of total surface of each residue that is exposed to water. All positions listed, except for 3.37, were accessible and protected in D2 receptor SCAM experiments (Javitch et al., 1995a,b, 1998; Fu et al., 1996). Receptor names are abbreviated according to their SWISS-PROT Annotated Protein Sequence Database entry names: http://www.expasy.ch/cgi-bin/lists?7tmrlist.txt
NR, SASA calculated without retinal; WR, SASA calculated with retinal; Δ, difference between NR and WR.
↵3-a G4157.42C in the D2 receptor did not bind ligand.